Analytical Chemiluminescence

Table of Contents

A Introduction

1. Electronic transitions and luminescence
2. Chemiluminescence spectroscopy

B Reagents

1. Luminol
2. Lophine and pyrogallol
3. Luciferins
4. Lucigenin and coelenterazine
5. Dioxetanes and oxalates
6. Organic peroxides and lipid peroxidation
7. Manganese
8. Cerium
9. Ruthenium
10. Oxygen radicals
11. Sulfites and persulfates
12. Hypohalites and halates

C. Enhancement of Chemiluminescence

1. Micellar enhancement
2. Dye enhancement
3. Enhancement by ultrasound

D. Instrumentation

1. Detection of chemiluminescence
2. Flow injection analysis (FIA)
3. Sequential injection analysis (SIA): lab on a valve
4. Lab on a chip
5. Chemiluminescence sensors
6. Chemiluminescence imaging
7. Electrochemiluminescence
8. Photo-induced chemiluminescence
9. Chemiluminescence detection in gas chromatography
10. Chemiluminescence detection in high performance liquid chromatography
11. Chemiluminescence detection in capillary electrophoresis

E. Applications

1. Environmental analysis
2. Pharmaceutical analysis
3. Food analysis
4. Biomedical analysis

The starting point for this wiki-book is a synopsis of analytical chemiluminescence, <http://myweb.tiscali.co.uk/chemiluminescence>. In its fifteen thousand words it does not attempt to summarise all the work done to date. It aims to use mainly well-established literature to give a brief introduction to the achievements of each division of the field. It deals with the research and development that underlies the present state of the art. The chemistry of each reagent that has been used in chemiluminescence is explained and the techniques for increasing the magnitude of the emitted signal are discussed. Further sections describe the different instrumental methods that have been used and examine the sort of work that has been carried out with them. The final sections discuss the applications of chemiluminescence in some of the major areas of analytical chemistry. The aim of the book is to give a comprehensive account of the subject suitable for students, research workers, professional analytical chemists and life scientists.