Proteomics/Protein Separations - Centrifugation/CENTRIFUGATION

1. Centrifugation
2. Some fundamental physical and mathematical concepts
3. Centrifugal fractionation of tissues and cells: Basic approaches and instrumentation
4. Density gradient centrifugation: Preparing gradients and measuring density
5. Density Gradient centrifuation: Rate and isopynic separations of particles

Centrifugation

Tabletop centrifuge

Centrifugation is one of the most important and widely applied research techniques in biochemistry, in cellular and molecular biology, and in medicine. In its various forms, centrifugation is regularly used to:

(1) remove cells or other suspended particles from their surrounding milieu on either a batch or a continous-flow basis.

(2)separate one cell type from another.

(3)isolate viruses and macromolecules, including DNA,RNA,proteins, and lipids or establish physical parameters of these particles fron their observed behavior during centrifugation.

(4)separate from dispersed tissue the various subcellular organelles including nuclei, mitochondria, cholorplasts, golgi bodies , lysosomes, peroxisomes, glyoxysomes, plasma membranes, endoplasmic reticulum , polysomes, and ribosomal subunits.

Reference: Centrifugation in Biology and Medical Science==Author Phillip sheeler, dept of biology , california state university, northridge, california