Proteomics/Protein Separations- Electrophoresis/Timelines of Electrophoresis

1. Gel Electrophoresis
2. One Dimensional Gel Electrophoresis
3. Two Dimensional Polyacrylamide Gel Electrophoresis(2D-PAGE)
4. Differential in Gel Electrophoresis(DIGE)
5. QPNC-PAGE
6. Capillary Electrophoresis
7. Timeline of Electrophoresis
8. Databases
9. Web Pages
10. Online Applications
11. Further Readings

Timeline of Electrophoresis

1930s: Use of sucrose for gel electrophoresis.

1937: The official launching of Tiselius' moving boundary electrophoresis (MBE).

1939: Zone Electrophoresis

1950: Rich in introducing novel methodologies in zone electrophoresis (ZE)

1950: Agar Gel electrophoresis

1955: Introduction of starch gels

1957: Cellulose acetate Electrophoresis (CAc) by Kohn

1959: Introduction of acrylamide gels by Raymond and Winstraub

1961: Electrophoresis in silica gel by Honnegar

1961: IsoElectricFocusing by Svensson

1964: Electrophoresis in thin layer of sephadex beads by Fasella et.al

1964: Disc gel electrophoresis Ornstein and Davis

1969: Introduction of denaturing agents especially SDS separation of protein subunit by Beber and Osborn

1970: T4 phage proteins were separated using stacking gel and SDS by Laemmli

1975: 2 Dimensional gels: Isoelectric focusing and SDS gel electrophoresis by OFarrell.

1977: Sequencing gels

1970s: agars gels

1980s: Immobilized pH gradients

1983: Pulsed field gel electrophoresis

1983: Capillary electrophoresis

References

• Electrophoresis: the march of pennies, the march of dimes. Righetti PG.
• http://www.csupomona.edu/~nebuckley/Courses/Bio451/Lectures/Bio451Lecture4.htm